Consulta: subjectFacets:"Cell proliferation"
Registros recuperados: 38
Data/hora: 19/05/2024 00:52:51

Alkaloid-rich fraction of Himatanthus lancifolius contains anti-tumor agents against leukemic cells
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Lima,Melissa Pires de; Hilst,Luciana Farhat; Mattana,Fernanda Vanessa Rechinbach; Santos,Cid Aimbiré de Moraes; Weffort-Santos,Almeriane Maria.
The effects of the alkaloid-rich fraction of Himatanthus lancifolius (Müll. Arg) Woodson on normal marrow cells and leukemic cell lines were investigated. After 48 h exposure, the proliferation assay showed significant cell growth inhibition for Daudi (0.1-10 µg/mL), K-562 (1-10 µg/mL), and REH cells (10-100 µg/mL), yet was inert for normal marrow cells. A similar inhibition profile was observed in clonogenic assays. This alkaloid-rich fraction, in which uleine is the main compound, showed no signs of toxicity to any cells up to 10 µg/mL. Cell feature analyses after induction of differentiation showed maintenance of the initial phenotype. Flow cytometric expression of Annexin-V and 7-AAD in K-562 and Daudi cells has indicated that the cells were not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apocynaceae; Cell proliferation; Himatanthus lancifolius; Indole alkaloids; Leukemia; Plant extract.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200014

Angiotensin-converting enzyme inhibition by lisinopril enhances liver regeneration in rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Ramalho,F.S.; Ramalho,L.N.Z.; Castro-e-Silva Júnior,O.; Zucoloto,S.; Corrêa,F.M.A.
Bradykinin has been reported to act as a growth factor for fibroblasts, mesangial cells and keratinocytes. Recently, we reported that bradykinin augments liver regeneration after partial hepatectomy in rats. Angiotensin-converting enzyme (ACE) is also a powerful bradykinin-degrading enzyme. We have investigated the effect of ACE inhibition by lisinopril on liver regeneration after partial hepatectomy. Adult male Wistar rats underwent 70% partial hepatectomy (PH). The animals received lisinopril at a dose of 1 mg kg body weight-1 day-1, or saline solution, intraperitoneally, for 5 days before hepatectomy, and daily after surgery. Four to six animals from the lisinopril and saline groups were sacrificed at 12, 24, 36, 48, 72, and 120 h after PH. Liver...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Angiotensin-converting enzyme inhibitors; Lisinopril; Bradykinin; Liver regeneration; Cell proliferation; Hepatocyte.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100016

Antiproliferative effect of urolithin A, the ellagic acid-derived colonic metabolite, on hepatocellular carcinoma HepG2.2.15 cells by targeting Lin28a/let-7a axis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Qiu,Zhenpeng; Zhou,Junxuan; Zhang,Cong; Cheng,Ye; Hu,Junjie; Zheng,Guohua.
An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively. Results revealed that urolithin A induced cytotoxicity in HepG2.2.15 cells, which was accompanied by the cleavage of caspase-3 protein and down-regulation of Bcl-2/Bax ratio. Moreover, urolithin A suppressed the protein expressions of Sp-1, Lin28a,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Urolithin A; Hepatocellular carcinoma; Lin28a; Let-7a; Cell proliferation; HBx.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700602

Antiproliferative effects of 1,25-dihydroxyvitamin D3 on breast cells: a mini review
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Bortman,P.; Folgueira,M.A.A.K.; Katayama,M.L.H.; Snitcovsky,I.M.L.; Brentani,M.M.
The hormone 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), the active form of vitamin D3, is an important regulator of calcium homeostasis, exerts antiproliferative effects on various cell systems and can induce differentiation in some kinds of hematopoietic cells. These effects are triggered by its receptor, vitamin D receptor (VDR), a phosphoprotein member of the nuclear receptor superfamily, which functions as a transcriptional factor. VDR binds as a heterodimer with retinoid X receptor (R X R) to hexameric repeats, characterized as vitamin D-responsive elements present in the regulatory region of target genes such as osteocalcin, osteopontin, calbindin-D28K, calbindin-D9K, p21WAF1/CIP1, TGF-ß2 and vitamin D 24-hydroxylase. Many factors such as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcitriol; Calcitriol receptor; Calcitriol analog; Breast tumor; Cell proliferation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100001

BC047440 antisense eukaryotic expression vectors inhibited HepG2 cell proliferation and suppressed xenograft tumorigenicity
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zheng,Lu; Liang,Ping; Zhou,JianBo; Huang,XiaoBing; Wen,Yu; Wang,Zheng; Li,Jing.
The biological functions of the BC047440 gene highly expressed by hepatocellular carcinoma (HCC) are unknown. The objective of this study was to reconstruct antisense eukaryotic expression vectors of the gene for inhibiting HepG2 cell proliferation and suppressing their xenograft tumorigenicity. The full-length BC047440 cDNA was cloned from human primary HCC by RT-PCR. BC047440 gene fragments were ligated with pMD18-T simple vectors and subsequent pcDNA3.1(+) plasmids to construct the recombinant antisense eukaryotic vector pcDNA3.1(+)BC047440AS. The endogenous BC047440 mRNA abundance in target gene-transfected, vector-transfected and naive HepG2 cells was semiquantitatively analyzed by RT-PCR and cell proliferation was measured by the MTT assay. Cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hepatocellular carcinoma; BC047440; Antisense eukaryotic expression vector; Cell proliferation; Tumorigenicity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200002

Biological activity and binding of estradiol to SK-Mel 23 human melanoma cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Sarti,M.S.M.V.; Visconti,M.A.; Castrucci,A.M.L.
Patients expressing estradiol receptors in melanoma cells have been reported to have a better prognosis. We therefore decided to investigate the in vitro effects of ß-estradiol and tamoxifen on the growth and tyrosinase activity of SK-Mel 23 human melanoma cells. Twenty-four-hour treatment with 0.4 nM ß-estradiol inhibited cell proliferation in 30% (0.70 ± 0.03 x 10(5) cells) and increased tyrosinase activity in 50% (7130.5 ± 376.5 cpm/10(5) cells), as compared to untreated cells (1.0 ± 0.05 x 10(5) cells and 4769 ± 25.5 cpm/10(5) cells, respectively). Both responses were completely (100%) blocked by 1 µM tamoxifen. Higher concentrations (up to 1.6 nM) or longer treatments (up to 72 h) did not result in a larger effect of the hormone on proliferation or...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: SK-Mel 23 cell line; Human melanoma; SS-estradiol; Tamoxifen; Cell proliferation; Tyrosinase activity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600016

Cell proliferation and migration in the jejunum of suckling rats submitted to progressive fasting
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gomes,J.R.; Alvares,E.P.
Cell proliferation and migration in the intestinal crypts, and cell migration in the villus are controlled by different mechanisms in adult rats. In the present study, weanling rats and fasting rats were used to quantitatively study the correlation of cell cycle parameters and epithelial cell migration in crypts and intestinal villi. Eighteen-day-old rats received a single injection of tritiated thymidine [3H]TdR (23:00 h); half of the pups were submitted to fasting 5 h earlier. Cell proliferation was determined in radioautographs of jejunal crypts, on the basis of the labeling indices (LI) taken 1, 8, 13 and 19 h after [3H]TdR. The results showed that the labeling index did not differ 1 h or 19 h after [3H]TdR between the fed (38.7% or 48%) and fasting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell proliferation; Cell migration; Crypt and villus intestine; Weanling rats; Radioautography.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200011

Cholesterol changes the fatty acid composition of rat enterocytes
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Gazzola,J.; Martins,E.F.; Miyasaka,C.K.; Palanch,A.C.; Vecchia,M.G.; Curi,R.
The effect of free cholesterol on the fatty acid composition and growth of rat fetal enterocytes was investigated in the absence and presence of 10% (v/v) fetal calf serum. Cholesterol caused a significant reduction of cell number after 6 and 12 h in culture. The fatty acid composition of enterocytes cultured in the presence of serum was also changed by the presence of 20 µM cholesterol. The fatty acid profile was determined by HPLC using fluorescence detection (325 nm excitation and 395 nm emission). Cholesterol (20 µM) increased the proportion (given in percentage of the total fatty acids) of the following fatty acids in cultured cells: lauric (by 42%), oleic (by 34%), linoleic (by 44%) and gamma-linolenic (by 20%) acids and reduced the proportion of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Enterocytes; Cholesterol; Fatty acid composition; Cell proliferation.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000100019

CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-κB pathway
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Long,T.Y.; Jing,R.; Kuang,F.; Huang,L.; Qian,Z.X.; Yang,T.L.
Myocardial ischemia is a major cause of death and remains a disease with extremely deficient clinical therapies and a major problem worldwide. Cold inducible RNA-binding protein (CIRBP) is reported to be involved in multiple pathological processes, including myocardial ischemia. However, the molecular mechanisms of myocardial ischemia remain elusive. Here, we first overexpressed CIRBP by transfection of pc-CIRBP (pcDNA3.1 containing coding sequenced for CIRBP) and silenced CIRBP by transfection of small interfering RNA targeting CIRBP (siCIRBP). pcDNA3.1 and the negative control of siCIRBP (siNC) were transfected into H9C2 cells to act as controls. We then constructed a cell model of myocardial ischemia through culturing cells in serum-free medium with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myocardial ischemia; CIRBP; NF-κB; Cell proliferation; Cell apoptosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400602

Development of potency assays for a plasmid containing vascular endothelial growth factor 2
Provedor de dados: Electronic Journal of Biotechnology
Autores: Huang,Li-chun; Chin,Emily; Chiang,Yawen L.
We have developed analytical methods to measure the biological functions of pVGI.1(VEGF2), a naked plasmid DNA product containing vascular endothelial growth factor 2 used in clinical trials for coronary artery diseases (CAD) and peripheral artery diseases (PAD). After being injected into muscles, vascular endothelial growth factor 2 (VEGF-2), presumably expressed in muscle tissues, binds to the endothelial cell receptors VEGFR2 or VEGFR3, triggering the downstream responses including cell proliferation and vascularization. As it is important to make sure clinical material is biological active, we developed a quantitative assay first to measure the receptor binding activity of the pVGI.1(VEGF2) gene product expressed by the transfected host cells, and then...
Tipo: Journal article
Palavras-chave: Cell proliferation; Plasmid DNA; Potency assays; Receptor binding; VEGF-2.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100001

Estrogen and progesterone promote breast cancer cell proliferation by inducing cyclin G1 expression
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Tian,J-M.; Ran,B.; Zhang,C-L.; Yan,D-M.; LI,X-H.
Breast cancer is the most common cause of cancer among women in most countries (WHO). Ovarian hormone disorder is thought to be associated with breast tumorigenesis. The present study investigated the effects of estrogen and progesterone administration on cell proliferation and underlying mechanisms in breast cancer MCF-7 cells. It was found that a single administration of estradiol (E2) or progesterone increased MCF-7 cell viability in a dose-dependent manner and promoted cell cycle progression by increasing the percentage of cells in the G2/M phase. A combination of E2 and progesterone led to a stronger effect than single treatment. Moreover, cyclin G1 was up-regulated by E2 and/or progesterone in MCF-7 cells. After knockdown of cyclin G1 in MCF-7 cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Estrogen; Progesterone; Cyclin G1; Cell proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300611

Evaluation of cell proliferation and apoptosis in placentas of rats with severe diabetes
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Rudge,Marilza Vieira Cunha; Costa,Elaine; Barbisan,Luis Fernando; Damasceno,Débora Cristina; Bueno,Aline; Saito,Felipe Hiroshi; Calderon,Iracema Mattos Paranhos; Rodrigues,Marcela Marcondes Pinto.
The aim of this work was to analyze the cell proliferation and apoptosis indexes on the 18th and 21st days of pregnancy of diabetic rats and to correlate with maternal glycemia and perinatal outcomes. Placentas from 20 Wistar rats were collected and divided into four experimental groups: control and diabetic of 18 and 21 days of pregnancy. The cell proliferation was analyzed using the PCNA expression and apoptosis by the TUNEL method. It was observed that PCNA and TUNEL indexes decreased from day 18 to 21 of pregnancy in the placentas of diabetic rats and these values were lower than control groups. Diabetic dams presented higher percentage of small for pregnancy age (SPA) fetuses. However, there was no difference between the PCNA and TUNEL indexes in SPA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diabetes; Cell proliferation; Apoptosis; Placenta; Rat; IUGR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200009

Evaluation of chromosomal abnormalities by cIg-FISH and association with proliferative and apoptotic indexes in multiple myeloma
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Linardi,C.C.G.; Martinez,G.; Velloso,E.D.R.P.; Leal,A.M.; Kumeda,C.A.; Buccheri,V.; Azevedo,R.S.; Peliçario,L.M.; Dorlhiac-Llacer,P.
Eighty-six newly diagnosed multiple myeloma (MM) patients from a public hospital of São Paulo (Brazil) were evaluated by cIg-FISH for the presence of del(13)(q14), t(4;14)(p16.3;q32) and del(17)(p13). These abnormalities were observed in 46.5, 9.3, and 7.0% of the patients, respectively. In order to identify the possible role of del(13)(q14) in the physiopathology of MM, we investigated the association between this abnormality and the proliferative and apoptotic indexes of plasma cells. When cases demonstrating t(4;14)(p16.3;q32) and del(17)(p13) were excluded from the analysis, we observed a trend towards a positive correlation between the proportion of cells carrying del(13)(q14) and plasma cell proliferation, determined by Ki-67 expression (r = 0.23, P...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Multiple myeloma; Cell proliferation; Apoptosis; Prognosis; Fluorescence in situ hybridization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100012

Experimental lamellar keratoplasty in rabbits using microfibrilar cellulose membrane: clinical, morphological and immunohistochemical findings
Provedor de dados: Ciência Rural
Autores: Macedo,Luciana Riacciardi; Ribeiro,Alexandre Pinto; Conceição,Luciano Fernandes da; Galera,Paula Diniz; Laus,José Luiz.
The clinical, histopathological and immunohistochemical features of the cornea were investigated in adult male New Zealand rabbits submitted to lamellar keratoplasty with microfibrillar cellulose membrane. Thirty animals were divided into five groups (n=6) and evaluated up to 60 days after surgery. Clinical examination revealed moderate manifestations of edema, blepharospasm and photophobia on the second day, which became mild or disappeared after the seventh day. This period was characterized clinically by repair of the corneal defect. Histopathological analysis showed the presence of a thin layer of squamous cells covering the damaged area as early as 7th day, accompanied by a mild infiltrate of polymorphonuclear cells. Blood vessels were observed in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corneal repair; Surgery; Cell proliferation; Ki67.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200019

Expression of immune-related genes in the oyster Crassostrea gigas during ontogenesis
Provedor de dados: Institutional Archive of Ifreme (ArchiMer)
Autores: Tirape, Ana; Bacque, Coralie; Brizard, Raphael; Vandenbulcke, Franck; Boulo, Viviane.
The work presented here reports the expression of immune-related genes during ontogenesis in the oyster Crassostrea gigar. Expression patterns of 18 selected genes showed that RNAs detected in oocytes and 2-4 cell embryos are of maternal origin and that gene transcription starts early after fertilization. The expression patterns of 4 genes (Cg-timp, Cg-tal, Cg-EcSOD and Drac3) suggested that hemocytes appear in the gastrula-trochophore stages. The localization of Cg-tal expression suggested that hematopoietic cells were derived from vessels and/or artery endothelia cells. Moreover, a bacterial challenge affected the level of expression of genes. Indeed, a change in expression levels was observed for Cg-LBP/BPI, Cg-timp, Drac3 and Cg-MyD88 genes in larval...
Tipo: Text
Palavras-chave: Rel/NF kappa B pathway; Cell proliferation; Hematopoiesis; Mollusk immunity; Hemocytes; Bivalve mollusk.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-2612.pdf

Feeding manipulation elicits different proliferative responses in the gastrointestinal tract of suckling and weanling rats
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Palanch,A.C.; Alvares,E.P.
Food deprivation has been found to stimulate cell proliferation in the gastric mucosa of suckling rats, whereas the weanling period has been reported to be unresponsive in terms of proliferative activity. In the present study we analyze regional differences in the effect of milk or food deprivation on cell proliferation of the epithelia of the esophagus and of five segments of small intestine in suckling, weanling and newly weaned Wistar rats of both sexes. DNA synthesis was determined using tritiated thymidine to obtain labeling indices (LI); crypt depth and villus height were also determined. Milk deprivation decreased LI by 50% in the esophagus (from 15 to 8.35%) and small intestine (from 40 to 20%) of 14-day-old rats. In 18-day-old rats, milk and food...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Developing rats; Milk deprivation; Cell proliferation; Crypt and villus size; Esophagus; Small intestine.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400015

Heparan sulfate and control of cell division: adhesion and proliferation of mutant CHO-745 cells lacking xylosyl transferase
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Franco,C.R.C.; Rocha,H.A.O.; Trindade,E.S.; Santos,I.A.N.; Leite,E.L.; Veiga,S.S.; Nader,H.B.; Dietrich,C.P.
We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells. We used both wild-type (CHO-K1) cells and a mutant (CHO-745) which is deficient in the synthesis of proteoglycans due to lack of activity of xylosyl transferase. Using different amounts of wild-type and mutant cells, little adhesion was observed in the presence of laminin and type I collagen. However, when fibronectin or vitronectin was used as substrate, there was an enhancement in the adhesion of wild-type and mutant cells. Only CHO-K1 cells showed a time-dependent adhesion on type IV collagen. These results suggest that the two cell lines present different adhesive profiles. Several lines of experimental...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: CHO cells; Extracellular matrix proteins; Proteoglycans; Glycosaminoglycans; Cell adhesion; Cell proliferation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800001

Heparan sulfate proteoglycans: structure, protein interactions and cell signaling
Provedor de dados: Anais da Academia Brasileira de Ciências
Autores: Dreyfuss,Juliana L.; Regatieri,Caio V.; Jarrouge,Thais R.; Cavalheiro,Renan P.; Sampaio,Lucia O.; Nader,Helena B.
Heparan sulfate proteoglycans are ubiquitously found at the cell surface and extracellular matrix in all the animal species. This review will focus on the structural characteristics of the heparan sulfate proteoglycans related to protein interactions leading to cell signaling. The heparan sulfate chains due to their vast structural diversity are able to bind and interact with a wide variety of proteins, such as growth factors, chemokines, morphogens, extracellular matrix components, enzymes, among others. There is a specificity directing the interactions of heparan sulfates and target proteins, regarding both the fine structure of the polysaccharide chain as well precise protein motifs. Heparan sulfates play a role in cellular signaling either as receptor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycosaminoglycans and protein interactions; Growth factors; Focal adhesion; Extracellular matrix; Cell cycle; Cell proliferation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300007

Immunomodulatory activity of Tityus serrulatus scorpion venom on human T lymphocytes
Provedor de dados: Journal of Venomous Animals and Toxins Including Tropical Diseases
Autores: Casella-Martins,Andrea; Ayres,Lorena R; Burin,Sandra M; Morais,Fabiana R; Pereira,Juliana C; Faccioli,Lucia H; Sampaio,Suely V; Arantes,Eliane C; Castro,Fabiola A; Pereira-Crott,Luciana S.
Abstract  Background Tityus serrulatus scorpion venom (TsV) contains toxins that act on K + and Na + channels and account for the venom’s toxic effects. TsV can activate murine peritoneal macrophages, but its effects on human lymphocytes have been poorly investigated. Considering that lymphocytes may play an important role in envenomation, we assessed whether TsV affects the expression of phenotypic (CD3, CD4, and CD8) and activation (CD69, CD25, and HLA-DR) markers, cell proliferation, and cytokine production in peripheral blood mononuclear cells.  Methods  Cytotoxicity of TsV was evaluated via the MTT assay. Cell proliferation, expression of phenotypic and activation markers, and release of cytokines were assessed using flow cytometry, after treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunomodulation; Immunophenotyping; T lymphocyte; Cell proliferation; Cytokine; Tityus serrulatus venom.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100350

In vitro investigations of Cynara scolymus L. extract on cell physiology of HepG2 liver cells
Provedor de dados: Brazilian Journal of Pharmaceutical Sciences
Autores: Löhr,Gesine; Deters,Alexandra; Hensel,Andreas.
The objective of this study was the investigation of a potential influence of artichoke leaf extract (ALE) on the cell physiology and gene expression of phase I/II enzymes of human liver cells HepG2 and investigation on potential cell protective effects against ethanol-induced cell toxicity against HepG2 cells. Cell biological assays under in vitro conditions using HepG2 liver cells and investigation of mitochondrial activity (MTT test), proliferation assay (BrdU incorporation ELISA), LDH as toxicity marker, gene expression analysis by RT-PCR and enzyme activity of glutationtransferase. Artichocke extract, containing 27% caffeoylquinic acids and 7% flavonoids induced mitochondrial activity, proliferation and total protein content under in vitro conditions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cynara scolymus L. Asteraceae; Artichoke; Liver cells; Cell proliferation; Gene expression.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200003

Kaempferol suppresses human gastric cancer SNU-216 cell proliferation, promotes cell autophagy, but has no influence on cell apoptosis
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,Fan; Ma,Cuimei.
Gastric cancer remains a serious threat to human health worldwide. Kaempferol is a plant-derived flavonoid compound with a wide range of pharmacological activities. This study aimed to investigate the effects of kaempferol on gastric cancer SNU-216 cell proliferation, apoptosis, and autophagy, as well as underlying potential mechanisms. Viability, proliferation, and apoptosis of SNU-216 cells after kaempferol treatment were evaluated using cell counting kit-8 assay, 5-btomo-2′-deoxyuridine incorporation assay, and annexin V-FITC/PI staining, respectively. Quantitative reverse transcription PCR was performed to measure the mRNA expressions of cyclin D1 and microRNA-181a (miR-181a) in SNU-216 cells. Cell transfection was used to down-regulate the expression...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gastric cancer; Kaempferol; MicroRNA-181a; Cell proliferation; Cell autophagy.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200610

Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro
Provedor de dados: Biological Research
Autores: Pan,Zeya; Pan,Hao; Zhang,Jin; Yang,Yun; Liu,Hui; Yang,Yuan; Huang,Gang; Ni,Junsheng; Huang,Jian; Zhou,Weiping.
BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells. Cell growth was significantly inhibited as determined by MTT assay. Crystal violet staining indicated that colony numbers and sizes were both reduced after...
Tipo: Journal article
Palavras-chave: Ubiquitin Specific Peptidase 39; Lentivirus; Human hepatocellular carcinoma; Cell proliferation; Cell cycle.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100018

Long non-coding RNA CHCHD4P4 promotes epithelial-mesenchymal transition and inhibits cell proliferation in calcium oxalate-induced kidney damage
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,C.; Yuan,J.; Hu,H.; Chen,W.; Liu,M.; Zhang,J.; Sun,S.; Guo,Z.
Kidney stone disease is a major cause of chronic renal insufficiency. The role of long non-coding RNAs (lncRNAs) in calcium oxalate-induced kidney damage is unclear. Therefore, we aimed to explore the roles of lncRNAs in glyoxylate-exposed and healthy mouse kidneys using microarray technology and bioinformatics analyses. A total 376 mouse lncRNAs were differentially expressed between the two groups. Using BLAST, 15 lncRNA homologs, including AU015836 and CHCHD4P4, were identified in mice and humans. The AU015836 expression in mice exposed to glyoxylate and the CHCHD4P4 expression in human proximal tubular epithelial (HK-2) cells exposed to calcium oxalate monohydrate were analyzed, and both lncRNAs were found to be upregulated in response to calcium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kidney calculi; Long non-coding RNAs; Fibrosis; Cell proliferation.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000100610

Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Pizarro,C.B.; Oliveira,M.C.; Coutinho,L.B.; Ferreira,N.P.
Pituitary adenomas sometimes show rapid growth and recurrence, and about one third invade the structures surrounding the sella turcica. In an attempt to determine aggressive behavior at an early stage, we used the MIB-1 antibody to identify the Ki-67 antigen. The present study was designed to evaluate pituitary adenomatous tissue in terms of secretion and proliferation and to correlate the Ki-67 index with hormone phenotype and invasive behavior. Material from 159 patients submitted to one or more resections of pituitary adenomas was evaluated. Forty-two non-secretory adenomas and 43 adenomas immunoreactive for growth hormone, 19 for prolactin, 18 for growth hormone and prolactin, 16 for adrenocorticotropic hormone (ACTH), and 21 cases of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pituitary adenoma; Invasive adenoma; Ki-67 antigen; MIB-1 antibody; Cell proliferation; Immunohistochemistry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200011

Modulation of IL-10/IL-10R expression by mafosfamide, a derivative of 4-hydroxycyclophosphamide, in a rat B-cell lymphoma
Provedor de dados: Biocell
Autores: Rico,María J.; Matar,Pablo; Scharovsky,O. Graciela.
We have already shown that IL-10 plays an important role in immunosuppression and metastatic dissemination in the rat B-cell lymphoma L-TACB model. It was suggested that the up-regulation of IL-10 production and IL-10 receptor (IL-10R) expression would be part of the transition from primary tumor to metastatic phenotype and that IL-10, besides its immunosuppressive activity, may act as a growth factor for metastatic L-TACB cells. The treatment of L-TACB-bearing rats with a single low-dose cyclophosphamide decreased IL-10 production, reverted immunosuppression and induced the immunologic rejection of tumor metastasis without any effect on primary tumor growth. Our current aim was to investigate the effects of cyclophosphamide on the expression of IL-10 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metastasis; Cyclophosphamide; Cell proliferation.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200006

Pancreatic β-cell function is inhibited by miR-3666 in type 2 diabetes mellitus by targeting adiponectin
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Tan,J.; Tong,A.; Xu,Y.
Type 2 diabetes mellitus (T2D) is a common endocrine and metabolic disorder, and poses threats to human health worldwide. Recently, microRNAs (miRNAs) have been suggested to play important roles in the pathophysiology of T2D. In this study, we explored the role of miR-3666 in T2D. miR-3666 was significantly down-regulated in the serum of T2D patients when compared to that of healthy volunteers, and miR-3666 expression level was negatively correlated with blood glucose levels of T2D patients. Overexpression of miR-3666 inhibited cell proliferation, reduced insulin secretion, and promoted cell apoptosis of pancreatic β-cell line (INS-1 cells). On the other hand, knockdown of miR-3666 had the opposite effects in INS-1 cells. The bio-informatics analysis using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Type 2 diabetes mellitus; MiR-3666; Insulin secretion; Cell proliferation; ADIPOQ.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000600607

Post-treatment with plant extracts used in Brazilian folk medicine caused a partial reversal of the antiproliferative effect of glyphosate in the Allium cepa test
Provedor de dados: Biocell
Autores: Dal-Souto Frescura,Viviane; Wouters Kuhn,Andrielle; Laughinghouse Iv,Haywood Dail; Terezinha Paranhos,Juçara; Bosio Tedesco,Solange.
Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared with the negative control (distilled water). Surprisingly, however, both extracts from P. brachypoda and P. birotula caused a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Psychotria brachypoda; Psychotria birotula; Cell proliferation; Apoptosis.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200002

Propofol inhibits proliferation, migration, and invasion but promotes apoptosis by regulation of Sox4 in endometrial cancer cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Du,Qing; Liu,Jia; Zhang,Xuezhi; Zhang,Xin; Zhu,He; Wei,Ming; Wang,Shilei.
Propofol is an intravenous sedative hypnotic agent of which the growth-inhibitory effect has been reported on various cancers. However, the roles of propofol in endometrial cancer (EC) remain unclear. This study aimed to explore the effects of propofol on EC in vitro and in vivo. Different concentrations of propofol were used to treat Ishikawa cells. Colony number, cell viability, cell cycle, apoptosis, migration, and invasion were analyzed by colony formation, MTT, flow cytometry, and Transwell assays. In addition, the pcDNA3.1-Sox4 and Sox4 siRNA plasmids were transfected into Ishikawa cells to explore the relationship between propofol and Sox4 in EC cell proliferation. Tumor weight in vivo was measured by xenograft tumor model assay. Protein levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endometrial cancer; Propofol; Cell proliferation; Apoptosis; Sox4; Wnt/β-catenin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400605

Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Paez-Pereda,M.; Kuchenbauer,F.; Arzt,E.; Stalla,G.K.
The extracellular matrix is a three-dimensional network of proteins, glycosaminoglycans and other macromolecules. It has a structural support function as well as a role in cell adhesion, migration, proliferation, differentiation, and survival. The extracellular matrix conveys signals through membrane receptors called integrins and plays an important role in pituitary physiology and tumorigenesis. There is a differential expression of extracellular matrix components and integrins during the pituitary development in the embryo and during tumorigenesis in the adult. Different extracellular matrix components regulate adrenocorticotropin at the level of the proopiomelanocortin gene transcription. The extracellular matrix also controls the proliferation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Integrins; Pituitary gland; Pituitary adenomas; Pituitary hormones; Cell proliferation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001000005

Regulation of semaphorin 4D expression and cell proliferation of ovarian cancer by ERalpha and ERbeta
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Liu,Y.; Hou,Y.; Ma,L.; Sun,C.; Pan,J.; Yang,Y.; Zhou,H.; Zhang,J.
Ovarian cancer is one of the most common malignancies in women. Semaphorin 4D (sema 4D) is involved in the progress of multiple cancers. In the presence of estrogen-like ligands, estrogen receptors (ERα and ERβ) participate in the progress of breast and ovarian cancers by transcriptional regulation. The aim of the study was to investigate the role of sema 4D and elucidate the regulatory pattern of ERα and ERβ on sema 4D expression in ovarian cancers. Sema 4D levels were up-regulated in ovarian cancer SKOV-3 cells. Patients with malignant ovarian cancers had significantly higher sema 4D levels than controls, suggesting an oncogene role of sema 4D in ovarian cancer. ERα expressions were up-regulated in SKOV-3 cells compared with normal ovarian IOSE80...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Semaphorin 4D expression; ERα; ERβ; Ovarian cancer; Cell proliferation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300603

REGγ is a strong candidate for the regulation of cell cycle, proliferation and the invasion by poorly differentiated thyroid carcinoma cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,M.; Gan,L.; Ren,G.S.
REGγ is a proteasome activator that facilitates the degradation of small peptides. Abnormally high expression of REGγ has been observed in thyroid carcinomas. The purpose of the present study was to explore the role of REGγ in poorly differentiated thyroid carcinoma (PDTC). For this purpose, small interfering RNA (siRNA) was introduced to down-regulate the level of REGγ in the PDTC cell line SW579. Down-regulation of REGγ at the mRNA and protein levels was confirmed by RT-PCR and Western blot analyses. FACS analysis revealed cell cycle arrest at the G1/S transition, the MTT assay showed inhibition of cell proliferation, and the Transwell assay showed restricted cell invasion. Furthermore, the expression of the p21 protein was increased, the expression of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: REGγ; PDTC; Cell cycle; Cell proliferation; SW579 cell line; Thyroid carcinoma.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500012

RNAi-mediated knockdown of pituitary tumor- transforming gene-1 (PTTG1) suppresses the proliferation and invasive potential of PC3 human prostate cancer cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Huang,S.Q.; Liao,Q.J.; Wang,X.W.; Xin,D.Q.; Chen,S.X.; Wu,Q.J.; Ye,G.
Pituitary tumor-transforming gene-1 (PTTG1) is a proto-oncogene that promotes tumorigenesis and metastasis in numerous cell types and is overexpressed in a variety of human tumors. We have demonstrated that PTTG1 expression was up-regulated in both human prostate cancer specimens and prostate cancer cell lines. For a more direct assessment of the function of PTTG1 in prostate tumorigenesis, RNAi-mediated knockdown was used to selectively decrease PTTG1 expression in PC3 human prostate tumor cells. After three weeks of selection, colonies stably transfected with PTTG1-targeted RNAi (the knockdown PC3 cell line) or empty vector (the control PC3 cell line) were selected and expanded to investigate the role of PTTG1 expression in PC3 cell growth and invasion....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PTTG1; Prostate cancer; Cell proliferation; Transwell invasion assay; Anticancer drug resistance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100001

Serum microRNA-30c levels are correlated with disease progression in Xinjiang Uygur patients with chronic hepatitis B
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Zhang,J.; Ma,J.; Wang,H.; Guo,L.; Li,J.
We aimed to investigate the potential role and mechanism of microRNA-30c (miR-30c) in the pathological development of chronic hepatitis B (CHB). The serum levels of miR-30c in hepatitis B virus (HBV) carrier Xinjiang Uygur patients with inactive, low-replicative, high-replicative and HBe antigen-positive CHB were investigated. HepG2 cells were co-transfected with pHBV1.3 and miR-30c mimic or inhibitor or scramble RNA. The effects of miR-30c dysregulation on HBV replication and gene expression, cell proliferation and cell cycle were then investigated. miR-30c was down-regulated in Xinjiang Uygur patients with CHB compared to healthy controls and its expression level discriminated HBV carrier patients with inactive, low-replicative, high-replicative and HBe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic hepatitis B; Disease progression; MicroRNA-30c; Hepatitis B virus replication; Cell proliferation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600601

Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
Provedor de dados: Biological Research
Autores: Yin,Lei-Miao; Wei,Ying; Wang,Wen-Qian; Wang,Yu; Xu,Yu-Dong; Yang,Yong-Qing.
BACKGROUND: BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In this study, we examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection using rat airway smooth muscle cells. RESULTS: The WST-1 measurements obtained from the combined detection were consistent with those obtained from the separate detection, which suggested that the addition of BrdU 2 h prior to the WST-1 analysis did not affect the WST-1 results. The BrdU measurements obtained from the...
Tipo: Journal article
Palavras-chave: BrdU; WST-1; Cell proliferation; Cell viability; Airway smooth muscle cells.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100069

Subcutaneous administration of ketoprofen delays Ehrlich solid tumor growth in mice
Provedor de dados: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Autores: Souza,C.M.; Auler,P.A.; Reis,D.C.; Lavalle,G.E.; Ferreira,E.; Cassali,G.D.
Ketoprofen, a nonsteroidal anti-inflammatory drug (NSAID) has proven to exert anti-inflammatory, anti-proliferative and anti-angiogenic activities in both neoplastic and non-neoplastic conditions. We investigated the effects of this compound on tumor development in Swiss mice previously inoculated with Ehrlich tumor cells. To carry out this study the solid tumor was obtained from cells of the ascites fluid of Ehrlich tumor re-suspended in physiological saline to give 2.5x106cells in 0.05mL. After tumor inoculation, the animals were separated into two groups (n = 10). The animals treated with ketoprofen 0.1µg/100µL/animal were injected intraperitoneally at intervals of 24h for 10 consecutive days. Animals from the control group received saline. At the end...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammation; Cell proliferation; Ketoprofen; Neoplasm.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501376

The Effect of High Temperature on Viability, Proliferation, Apoptosis and Anti-oxidant Status of Chicken Embryonic Fibroblast Cells
Provedor de dados: Revista Brasileira de Ciência Avícola
Autores: Ibtisham,F; Zhao,Y; Nawab,A; Liguang,H; Wu,J; Xiao,M; Zhao,Z; An,L.
ABSTRACT The effects of oxidative stress induced by high temperature on the cell viability, proliferation, apoptosis and oxidative status of chicken embryonic fibroblasts (CEF) were analyzed. The viability, proliferation, apoptotic and anti-oxidative status were measured after incubating CEF at the temperatures of 37ºC (control) and 40-44ºC (experimental groups) for 6,12 and 24 hours. The results showed that at high temperature (42-43ºC), the viability of CEF cells decreased after 6, 12 and 24 h of incubation, but the difference was significant only at 43ºC. Cell proliferation was significantly reduced at 44oC/6h. The apoptotic rate of CEF cells was increased following heat treatments in a time-dependent manner. ROS formation increased with increasing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; Chicken embryonic fibroblast; Cell proliferation; Heat stress; Oxidative stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300463

The effect of losartan on angiotensin II-induced cell proliferation in a rat aorta smooth muscle cell line
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Tambelline,Natália; Oliveira,Karen; Olchanheski Junior,Luiz Renato; Sordi,Regina; Otuki,Michel Fleith; Favero,Giovani Marino; Fernandes,Daniel.
The success of revascularization procedures is limited by recurrent stenosis, which is a narrowing of a blood vessels that results from neo-intimal hyperplasia. The renin-angiotensin-aldosterone system has been implicated in the pathogenesis of neo-intimal hyperplasia, and a role for angiotensin II in vascular smooth muscle cell proliferation has been proposed. There are at least two high-affinity subtypes of angiotensin II receptors, AT1 and AT2, both of which are seven-transmembrane G protein-coupled receptors. We investigated the effect of losartan, an AT1 receptor antagonist, on vascular smooth muscle cell proliferation using the A7r5 smooth cell line derived from rat aorta. Losartan was shown to prevent angiotensin II-induced cell proliferation,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Losartan; Cell proliferation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200012

The role of calcium, calcium-activated K+ channels, and tyrosine/kinase in psoralen-evoked responses in human melanoma cells
Provedor de dados: Brazilian Journal of Medical and Biological Research
Autores: Isoldi,M.C.; Pereira,E.A.; Visconti,M.A.; Castrucci,A.M.L.
8-Methoxy psoralen (8-MOP) exerts a short-term (24 h) mitogenic action, and a long-term (48-72 h) anti-proliferative and melanogenic action on two human melanoma cell lines, SK-Mel 28 and C32TG. An increase of intracellular calcium concentration was observed by spectrofluorometry immediately after the addition of 0.1 mM 8-MOP to both cell lines, previously incubated with calcium probe fluo-3 AM (5 µM). The intracellular Ca2+ chelator BAPTA/AM (1 µM) blocked both early (mitogenic) and late (anti-proliferative and melanogenic) 8-MOP effects on both cell lines, thus revealing the importance of the calcium signal in both short- and long-term 8-MOP-evoked responses. Long-term biological assays with 5 and 10 mM tetraethylammonium chloride (TEA, an inhibitor of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 8-[Methoxy]-psoralen; SK-Mel 28 cell line; C32TG cell line; Cell proliferation; Cell signaling; Melanoma.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400013